Skip to Content

Herbicide-Hunting Bacteria

Modified bacteria seek out and metabolize a harmful pollutant.

Common lab bacteria have been turned into scavengers that seek and destroy the herbicide atrazine, an environmental pollutant that can be harmful to wildlife. Key to the transformation is the combination of a synthetic switch that allows the bacteria to chase the chemical and a gene taken from another species of bacteria for breaking down atrazine.

Some wild bacteria have evolved the ability to metabolize atrazine. Using a synthetic biology approach, a team at Emory University in Atlanta has now equipped a synthetic strain of E. coli with the ability to hunt down atrazine and metabolize it.

Bacteria normally use sensory proteins called chemoreceptors to spot chemicals in their environment. Reengineering one of these receptors into a designer protein that recognizes atrazine would have been a formidable challenge. So Justin Gallivan and his team instead turned to RNA to develop an atrazine-binding molecule called a riboswitch.

“A riboswitch is a piece of RNA that binds to a small molecule and changes shape when it does that, which then leads to a change in gene expression,” explains Gallivan. His group used a novel selection process to synthesize and evolve a new riboswitch from scratch in the lab. Coupling the riboswitch to a gene that controls movement allows bacteria to move toward nearby atrazine.

The team synthesized a quadrillion (10^15) pieces of RNA, each with a randomly ordered sequence of 40 nucleotides, and tested their ability to stick to atrazine. After repeating this for several rounds, and removing all the RNAs that bound the breakdown product of atrazine, the researchers had selected a much smaller selection of sequences that all stuck to atrazine.

The riboswitch also needs to be able to change shape in such a way that it only allows the protein to move when atrazine is present. Gallivan’s team fused the atrazine-binding sequences to another large selection of random RNA sequences, each a potential candidate for switching shape in just the right way. They then placed the whole package into E. coli bacteria, and checked which bacteria showed the ability to move when atrazine was present.

The bacteria that passed this test all turned out to carry the same switch sequence. Through further biochemical analysis of the RNA, Gallivan’s team showed that the switch works by preventing the cell’s protein production machinery from accessing the movement gene’s messenger RNA unless atrazine binding changes the shape of the switch and thereby frees the access point.

In the final step, the team also equipped the switch-carrying bacteria with an atrazine-degrading gene isolated from a different bacterium species. The resulting bacteria demonstrate their seek-and-destroy behavior by forming rings in petri dishes covered with atrazine as they move toward the atrazine and clear it from the plate.

Gallivan admits that there are several hurdles to overcome before his reprogrammed bacteria could clear up atrazine pollution in the field. For once, the cells get stuck once they have eaten all the atrazine. This could be tackled by reengineering the switch so that bacteria stop moving once they encounter atrazine and start again once they have cleared it. The system might also have to be transplanted into hardier bacteria that are able to survive in the harsh conditions at polluted sites, and further modifications might be necessary to improve the sensitivity to atrazine.

John Simon of the international consulting firm WSP Environment & Energy says that even with increased sensitivity, “probably the greatest application for such a biological remediation approach for atrazine would be at the location where the chemical is manufactured or managed in concentrated form–because otherwise the area is so extensive that it would be difficult to do economically.”

Simon expects that any field application is a long way off due to regulatory concerns about genetically modified organisms.

Víctor de Lorenzo, of the Molecular Environmental Microbiology Laboratory in Madrid, Spain, shares this concern, but believes that using synthetic biology to equip genetically modified organisms with tightly controlled specific functions is a good way to address safety issues. “This is an incredibly exciting demonstration of how you can rewire and effectively program bacteria to make them behave in the way that you want,” he says.

Warren Dick, a professor of soil science at Ohio State University, say that the approach “is very interesting and certainly has potential for increased removal from atrazine-contaminated sites.”

Keep Reading

Most Popular

Large language models can do jaw-dropping things. But nobody knows exactly why.

And that's a problem. Figuring it out is one of the biggest scientific puzzles of our time and a crucial step towards controlling more powerful future models.

OpenAI teases an amazing new generative video model called Sora

The firm is sharing Sora with a small group of safety testers but the rest of us will have to wait to learn more.

Google’s Gemini is now in everything. Here’s how you can try it out.

Gmail, Docs, and more will now come with Gemini baked in. But Europeans will have to wait before they can download the app.

This baby with a head camera helped teach an AI how kids learn language

A neural network trained on the experiences of a single young child managed to learn one of the core components of language: how to match words to the objects they represent.

Stay connected

Illustration by Rose Wong

Get the latest updates from
MIT Technology Review

Discover special offers, top stories, upcoming events, and more.

Thank you for submitting your email!

Explore more newsletters

It looks like something went wrong.

We’re having trouble saving your preferences. Try refreshing this page and updating them one more time. If you continue to get this message, reach out to us at customer-service@technologyreview.com with a list of newsletters you’d like to receive.