Protecting Neurons from Parkinson's Disease

Insights into the disease's protein culprit could lead to human therapies.

Researchers have uncovered a way to protect neurons from degeneration and death in animal models of Parkinson’s disease. Although scientists have known which protein is the main culprit in Parkinson’s nerve damage, both the protein's normal function and details of its harmful effects have remained a mystery. Now a recent genetic study has uncovered some of this protein’s mysterious activities, and researchers have used what they learned to save neurons affected by the disease. The research could lead to targeted therapies for human Parkinson’s.

Yeast cells have been engineered to grow fluorescently labelled clumps (brighter green areas) of a human Parkinson’s protein. The cells are being used to search for genes that protect against the disease. (Courtesy of Aaron Gitler, Whitehead Institute.)

A team of researchers, led by Susan Lindquist, an MIT biology professor and member of the Whitehead Institute for Biomedical Research, where the study was done, performed genetic screens in a yeast model to illuminate the role of the protein synuclein, which is present in large clumps in neurons affected by Parkinson’s. The disease’s characteristic tremors and muscle rigidity are caused by damage and death to a class of neurons that produce the neurotransmitter dopamine. Lindquist’s lab engineered yeast that make synuclein and that can act as a model for these neurons. Using these yeast, they screened large libraries of genes in search of those that counteracted synuclein’s toxicity.

Out of several candidate genes, one stood out: a gene involved in protein trafficking. Linquist’s group discovered that large amounts of misfolded synuclein seem to interfere with cells’ ability to shuttle proteins between two organelles. And these organelles play a critical role in refining proteins: before being shipped off to different parts of the cell, protein strings often need to be cut, folded into three-dimensional shapes, or have additional groups such as carbohydrates added. During this process, they are sheltered within a protective lipid bubble.

The researchers aren’t sure exactly how synuclein disrupts protein trafficking, but they suspect it disturbs these protective bubbles. “Dopamine must be packaged in these membranes and sequestered from [the insides of the cell], where it can cause oxidative damage,” says Aaron Gitler, a post-doc in Lindquist’s lab. He and Lindquist suggest that one reason dopamine-producing neurons die in Parkinson’s patients is that synuclein leaves them unprotected.

Using their gene-screening technique, Whitehead scientists found a way to interfere with overactive synuclein. When they activated the gene Rab 1, whose product is involved in shepherding freshly made proteins from one part of the cell to another, the Parkinson’s yeast lived. The equivalent protein in nematode, fly, and rat neurons also countered synuclein’s toxicity, suggesting that this protein helps neurons to overcome synuclein’s block in protein processing.

“The findings hold up nicely,” says Darren Moore, assistant professor at Johns Hopkins University School of Medicine’s Institute for Cell Engineering. The gene may also prove to be protective in human Parkinson’s. “Potentially drugs or gene therapy could be used to increase protein trafficking in human neurons,” says Gitler.