The footage could lead to novel techniques to block the transmission of HIV.
For the first time, researchers filmed the transfer of HIV
from infected to uninfected T cells through structures called virological
synapses. The study, conducted by researchers at Mount Sinai
School of Medicine, in New York, NY, and the Center for Biophotonics Science and Technology, University of California,
Davis, could lead to new methods to block the transmission of HIV. The study published
in the March 27 edition of Science.
Researchers captured the video, shown below, by creating a
molecular clone of infectious HIV that contains green fluorescent protein. They
then used quantitative, high-speed 3-D video microscopy to
record both viral particle formation and transmission of the virus between T
cells.
From the press release:
The resulting images and videos show that, once an infected cell
adheres to a healthy cell, the HIV proteins--which appear bright green in the
study--migrate within minutes to the contact site. At that point, large
packets of virus are simultaneously released by the infected cell and
internalized by the recipient cell. This efficient mode of transfer is a
distinct pathway from the cell-free infection that has been the focus of most
prior HIV studies, and reveals another mechanism by which the virus evades
immune responses that can neutralize free virus particles within the body.
"We found that the transfer of HIV is highly coordinated between T
cells, and that the transfer is rapid and massive," said [Benjamin] Chen, [assistant professor
of medicine, infectious diseases, Mount Sinai School of Medicine]."Future efforts
to block HIV transmission may be designed to specifically exploit and block
this cell-to-cell mode of infection."
A rotating three-dimensional view of an HIV-infected T cell (green) forming virological synapses with three healthy CD4+ cells (red). The viral structural protein, shown in green, accumulates in button-shaped structures at the contact sites.
This movie shows high-speed imaging of HIV transfer across a virological synapse. The video begins with a still view of two T cells with transmitted light and an outline of a spot where a synaptic button has formed between them. The movie then shows the movement of fluorescent viral protein into the synaptic button, followed by the transfer of material from the button into the target cells. Note that the movie focuses on the movement of the viral protein without indicating the outlines of the recipient cell.
The video shows a cell that has engaged in a synapse becoming infected days later. Infection was captured by continuous long-term imaging over three days. The HIV green fluorescence image is presented on the left while an overlay of green fluorescence and bright field image is shown on the right. Images were acquired every 10 minutes over 67 hours.
Video credit: © Science/AAAS
Massachusetts Institute of Technology
Comments
ryanvice
03/29/2009
Posts:1
then where’s the problem, attaching a killer to those fluorescent molecules?
smithsomian
03/29/2009
Posts:71
See http://jvi.asm.org/cgi/content/abstract/81/22/12596?ijkey=4e950db670614f62a085ff681ce2d1e068ec6fb4&keytype2=tf_ipsecsha
smithsomian
03/29/2009
Posts:71
Unfortunately, they will still carry the virus and still be able to infect people, except they will never contract AIDS and live to a very old age...
jmndos
03/29/2009
Posts:1
THE IDEA that AMBUSH cures AIDS
is being proven by the more than 400 individuals who have taken a dose of 60 ml three times daily for 21 days. The result is that AMBUSH 'KILLS' the virus by causing the protein envelope to rupture and the viral particles are discarded by the white blood cells. AMBUSH is able to 'KILL' the virus that are 'hiding' in the lymph system by its 'natural radioactive' properties. This process allows the body to 'return to normal health' with a corresponding immunity to that or those strains of the virus.
What is AMBUSH ?
AMBUSH is a radioactive isotope of uranium that is found in the 'palm' plant of which there are more than 3000 species. When ingested, AMBUSH causes the body temperature in the trunk area to rise to about 102 degrees when the individual is sleeping. The preparation takes four hours per batch, which is then given to the individuals for consumption 60 ml three times daily for 21 days. AMBUSH is a herbal preparation in this form but it contains an active ingredient which is a 'NEW' crystalline substance, a drug from the 'palm plant' similarly to ASPIRIN originating from the willow tree bark
RESULTS:
After 21 days on AMBUSH, ALL the individuals experienced a decrease in viral load to undetectable, an increase in cd4, increase in RBC, an improvement in general health such as more color to the face, decrease in Buffalo hump, an increase in gluteal muscles, a decrease to having no joint pains whereby individuals can bend to touch their toes, and walk up steps are but a few examples. There is also a dramatic increase in their sexual appetite beginning after the first week of therapy
DISCUSSION:
In any plant concoction such as percolated 'tea', there are 30-40,000 compounds, whi ch would take the scientific community twenty years to isolate one particular ingredient if they knew what they were looking for. The LORD GOD has given me seven steps to isolate the active ingredient, which is soft and metallic in nature and has a carbon- uranium-sulfur-(classified)-phentolamine configuration or structure. This is similar to Federick Kekule and the discovery of the benzene ring where he dreamt the structure.
As an antiviral and 'natural radioactivity' producing agent, AMBUSH is also effective against leukemia, lupus and HPV. Here I am saying that I have 'GIVEN' AMBUSH in the same 'strength' and dosage to patients with leukemia, lupus and HPV. A 35 year old male with HIV found it difficult to impossible to urinate was put on 'green tea' and water while the doctors contemplated prostrate surgery. One of the doctors gave him my number , I sent him a supply of AMBUSH an d he has not been given any more ARV's, since taking AMBUSH 18 months ago, is in 'good' health and has expressed a willingness to be examined by HIV investigators like many others who have taken AMBUSH.
I have sent this 'IDEA' to most HIV research agencies, scientist of the field, universities, hospitals, clinics, politicians and news agencies to which it is REJECTED because the name of THE LORD GOD is mentioned. He has steered me scientifically through the processes such as which plant and how to produce the active ingredient. What are the odds of a Florida Pharmacist picking a plant would contain the CURE for HIV/AIDS ?
I have never charged any of the people for their supply of AMBUSH but a life saving has been spent on the project with NO renumeration from any sources because AMBUSH falls outside the walls of modern medicine and research.
PROPOSAL:
My proposal is that I PROVE that AMBUSH CURES HIV/AIDS by giving it to a number of END-STAGE or DRUG-RESISTANT people and the scientific community watches their recovery. This proposal addresses the problem in that I have already outlaid the results to be obtained.
This IDEA is unconventional in that the scientific community has rejected AMBUSH because I say it is GOD given. Secondly if I wrote it according to certain standards, then it might be peer reviewed. However, THE LORD GOD has also shown me that there are five enzyme systems associated with the virus, reverse transcriptase, protease, fusion and two more of which causes the virus to be AIRBOURNE. This means that without DIVINE intervention mankind and ALL warm- blooded mammals will be extinct in a number of years.
The PROOF of what I am saying is found in scientific papers wherein it is found that when the protease cuts the viral strands, it cuts it at DIFFERENT lengths EVERY time, to which it should always be a valine at the end but is a different amino acid every time. This is why it is IMPOSSIBLE to produce a VACCINE.
Since this is NOT a hypothesis but there are about 400 individuals who have taken AMBUSH, here lies a vast area in which to check, recheck and confirm that AMBUSH CURES AIDS. Let it be mentioned that during the HIV reproductive cycle, reverse transcriptase converts viral RNA into DNA compatible to human genetic materials. Thus the human DNA has been 'hijacked' and since each person has a DIFFERENT DNA, then the new viral copy is unique to that person which shows that each individual has a DIFFERENT STRAIN of the virus. Consider two HIV positive people swapping viral strains and increasing its complexity with multiple partners.
It can also be proposed that they be revisited as proof that the strain or strains that they had were 'killed' at the time of taking AMBUSH considering that a person can catch as many different strains as there are people who are infected by HIV.
I am also willing to work with the scientific community in identifying those individuals who took AMBUSH and wish to be identified with this process notwithstanding that some are stigmatized while others are jubilant,
Once AMBUSH is verified as being able to accomplish that which is aforementioned then the next stage might be the natural and artificial synthesis of the substance.
Finally, if this is accepted or not, believed or not, THE LORD GOD always wins and this is the heavenly truth to which AMBUSH was divinely given to mankind for the CURE of HIV/AIDS and it will be here forever. Apostle Shada Mishe.
apostleshadamishe@gmail.com
Here is a video taped presentation that I gave at t he Martin Luther King library in Washington
http://www.youtube.com/watch?v=8V53D1w__Po
http://www.youtube.com/watch?v=vPwuwlVBOV0
http://www.youtube.com/watch?v=ZejptOwMTzQ
http://www.youtube.com/watch?v=CqcTgIAhrhc
http://www.youtube.com/watch?v=f7HPKcT_iwY
http://www.youtube.com/watch?v=W9iQfgiYAnw
http://www.youtube.com/watch?v=i3RzRS6tJDM
apostleshada...
04/06/2009
Posts:3
http://blog.insidecytolin.com/?p=197
sheather
10/13/2009
Posts:1
k syntax:
http://www.infectiousdiseasenews.com/article/44822.aspx
cbanks1
10/27/2009
Posts:1