An imaging system developed by a Massachusetts company makes visible the chemical activity of tissues deep inside the body. Researchers are using the system to watch the rise and spread of cancer in mice, and pharmaceutical companies are using it to better determine the effects of new drugs in animals. Made by VisEn Medical of Woburn, the molecular imaging system is allowing researchers to see deeper into the body and look at a wider range of chemical activity than is possible with existing imaging techniques.

Conventional imaging techniques, such as magnetic resonance imaging, give only anatomical information--they allow doctors to see the shape and size of tumors, for example. Researchers hope new molecular imaging technologies that provide information on the activity of tissues, as VisEn's technology does, will help doctors diagnose diseases earlier, monitor whether treatments are working, and even provide guidance during surgery. (See "Watching Cancer Cells Die" and "Seeing Tumors with Quantum Dots.")

The VisEn molecular imaging system relies on large fluorescent-protein probes that interact with disease-related proteins in the body and allow researchers to see where they are and in what concentrations. Some of these probe proteins constantly emit fluorescent light. Others, which provide clearer images, don't emit any light when they're injected in the body but light up when they are cleaved by specific proteins in the body. One probe protein detects an enzyme that helps tumors "chew up surrounding tissue" so that they can grow and spread, says Jeff Peterson, director of applied biology at VisEn. Other probes include one that detects a protein implicated in the bone changes associated with cancer and arthritis.

By measuring the intensity of fluorescence from the probes, researchers can find disease sites. Each probe fluoresces at a different wavelength, so it's possible to look for many different kinds of activities at once. For example, a researcher might look for bone-building and tissue-chewing proteins in breast tumors to determine how aggressive they are.

Because molecular imaging allows researchers to watch tissue activity in real time in live animals, "you can understand earlier if you're really modifying a disease with a drug," rather than just controlling symptoms, Peterson points out. He says that a pilot study with a pharmaceutical company demonstrated that for arthritis, imaging inflammatory proteins in live animals works as well as examining tissue from dead animals under the microscope. VisEn is collaborating with Merck, Eli Lilly, and other pharmaceutical companies.