Select your localized edition:

Close ×

More Ways to Connect

Discover one of our 28 local entrepreneurial communities »

Be the first to know as we launch in new countries and markets around the globe.

Interested in bringing MIT Technology Review to your local market?

MIT Technology ReviewMIT Technology Review - logo

 

Unsupported browser: Your browser does not meet modern web standards. See how it scores »

n the early days of bio-tech, all eyes focused on the techniques of “recombinant DNA”: splicing together bits of DNA from different sources. These 30-year-old genetic engineering methods are now the basis of a multibillion-dollar market for protein-based drugs. Today, another class of biotech drugs is emerging from the lab, but the technology for these “antisense therapies” isn’t new-it dates back to 1978, just a few years after the first gene-splicing experi-ments. A few persistent researchers have shepherded it down a long, bumpy road.

In the early 1970s, Paul Zamecnik (pronounced ZAM-es-nick) was studying a cancer-causing chicken virus that transmits its genetic information via RNA, a chemical cousin of DNA. Zamecnik and his colleagues at Massachusetts General Hospital found that, as the virus replicated, its RNA looped around on itself. They speculated that if they could block this step, they could stop the bug in its tracks. So they constructed a short piece of DNA designed to stick to the virus’s single strand of RNA and thereby gum up its works. The RNA encoded the virus’s proteins; functionally, it made sense, so the researchers called it the “sense” strand. The DNA molecule (called an oligo-nucleotide) was its chemical opposite-the “antisense.” Zamecnik mixed the designer DNA snippet with infected chicken cells, and voil-no cancer. He and colleague Mary L. Stephenson suggested that antisense molecules could be used to treat all sorts of infections-as well as cancer-by preventing RNA from being translated into the proteins the invaders need to live.

When the work appeared in the January 1978 Proceedings of the National Academy of Sciences, no one believed the experiment had worked. “It had been…a dogma that oligonucleotides didn’t get into cells,” Zamecnik says. The work languished in obscurity until the mid-1980s, when technological advances made the experiments easier to repeat. As biochemists began to see anti-sense as a magic bullet, companies sprang up to capitalize on the “new” technology. It wasn’t smooth sailing-difficulties with stability and specificity to targeted RNAs hindered its adoption. But now the technique seems ready to pay off. In 1998, the U.S. Food and Drug Administration approved the first antisense drug-a therapy for eye damage caused by cytomegalovirus. More than 20 other antisense drugs, most targeting cancer and viral infections, are in clinical trials. And Zamecnik, now nearly 90 years old, is still researching antisense treatments for drug-resistant forms of tuberculosis and malaria.

0 comments about this story. Start the discussion »

Tagged: Biomedicine

Reprints and Permissions | Send feedback to the editor

From the Archives

Close

Introducing MIT Technology Review Insider.

Already a Magazine subscriber?

You're automatically an Insider. It's easy to activate or upgrade your account.

Activate Your Account

Become an Insider

It's the new way to subscribe. Get even more of the tech news, research, and discoveries you crave.

Sign Up

Learn More

Find out why MIT Technology Review Insider is for you and explore your options.

Show Me
×

A Place of Inspiration

Understand the technologies that are changing business and driving the new global economy.

September 23-25, 2014
Register »