Technology Review - Published By MIT
Advertisement

Better Bug to Make Cellulosic Ethanol

Continued from page 1

By Prachi Patel

Tuesday, September 09, 2008

smaller text tool iconmedium text tool iconlarger text tool icon

However, the genetically engineered bacteria cannot break down cellulose. In their laboratory experiments, Lynd and his coworkers needed to add enzymes to free the glucose from crystals of cellulose. Still, the bacteria offer an advantage because they are thermophilic--that is, they naturally grow at temperatures of 50 to 60 ºC. This is much higher than the 37 degrees at which yeast ferments sugars, and thus the bacteria require less fewer enzymes. "Because enzymes are more active at higher temperatures, using these bacteria would mean you have to add less enzyme," Lynd says.

In the experiments, the bacteria fermented sugar mixtures at 50 ºC to give 4 percent ethanol concentration. "It's the highest concentration of ethanol that's been produced by thermophilic bacteria," Lynd says.

Conventional yeast can give higher ethanol concentrations of 10 to 12 percent, says Harvey Blanch, a chemical-engineering professor at the University of California, Berkeley. Nevertheless, he says that the new work is a "nice proof of concept" for a combined approach to make cellulosic ethanol. While the researchers use cellulose crystals in their lab experiment, the challenge will be to see if the microbes can produce similar results with cellulosic biomass such as wood chips and switchgrass, says Blanch. "If this can be successfully accomplished, it will be a significant advance," he says.

Lynd's team is also trying to increase ethanol yield in a thermophilic bacteria that breaks down cellulose. The group wants to team it with the bacteria that are good at breaking down hemicellulose and using all sugars. That would give an all-in-one microbe system that breaks down biomass and converts all of its sugars into ethanol.

"Using one microbe or community of microbes for essentially the whole conversion process would be a major cost breakthrough," says Anna Palmisano, associate director of science at the DOE's Office of Biological and Environmental Research. "It's one of the ways really fundamental biology could transform the equation and help pave the way to commercially viable cellulosic biofuels."

Comments

  • Not Ethanol
    Why not make propanol or some fuel other than ethanol that isn't corrosive?
    Rate this comment: 12345

    RD
    09/09/2008
    Posts:125
    Avg Rating:
    3/5
    • Re: Not Ethanol
      So you can drink & drive :P
      Rate this comment: 12345

      CountZ3ro
      09/09/2008
      Posts:20
      Avg Rating:
      2/5
    • Re: Not Ethanol
      I'm not sure, but I think:
      1) ethanol is less toxic, so the microbes can make more;
      2) the microbes naturally make ethanol; and
      3) once you have ethanol, it isn't too hard to make propanol just using chemistry.
      Everyone feel free to correct me.
      Rate this comment: 12345

      dmm
      09/09/2008
      Posts:207
      Avg Rating:
      3/5
  • Inaccuracies
    This article is inaccurate in several key ways, which can be misleading.

    First a minor point. Corn ethanol does not account for most ethanol production. It accounts for virtually all of it. Your use of "most" could mislead readers to think that cellulosic ethanol claims a significant share of production.

    More importantly, cellulosic ethanol processes comprise more than two substantive steps, meaning that this use of microbes would not reduce production to a simple "biomass-in, ethanol-out" process. Again, that's too rah-rah and misleading. Pretreatment is an important step for its costs and inefficiencies, including the loss of about half of the available cellulose. Finally distillation has huge energy costs of its own. There are no cellulosic processses (to my knowledge) that produce higher than a 4% solution. This requires three times the distillation of corn ethanol.

    It's also quite inaccurate to suggest that this process would work equally well on all the proposed cellulosic feedstocks. Experience with virtually every experimental process has shown relative success with just one or two feedstocks, meaning that separate technologies must be provided to make full use of them all.

    Pretreatment and distillation are two deal-breakers for cellulosic ethanol. The amounts and costs of enzymes have been another, though this is one area of improvement. The article mentions that use of the microbes reduces the need for enzymes. Does anyone know a rough figure for this reduction yet? Is it 5%, or is it much better?

    Finally, it's worth pointing out the dangers of mass-producing genetically engineered microbes for use in uncontrolled settings. Some such microbes have been recently approved for field tests but ended up being virulent to crops. It's a good thing the mistake was caught before the field test. The researcher believes it could have destroyed vast areas of farmland.

    Many of the microbes under study commonly grow within humans and livestock. E. Coli is one example. Do we want to produce a new version of E. Coli having new capabilities to feed on a wide variety of sugars and so on, then possibly have those E. Coli populate our intestines? I personally vote against that.

    I've said it many times. The most efficient and cost effective way to use biomass for energy is to burn it for electricity. If it's too expensive to do that, then it will be too expensive for anything.
    Rate this comment: 12345

    MakeSense
    09/09/2008
    Posts:99
    Avg Rating:
    3/5
    • Re: Inaccuracies

      I've said it many times. The most efficient and cost effective way to use biomass for energy is to burn it for electricity. If it's too expensive to do that, then it will be too expensive for anything.

      Bingo. It can't be said any better than that.
      Rate this comment: 12345

      beester
      09/10/2008
      Posts:1
      Avg Rating:
      3/5
    • Re: Inaccuracies
      "The most efficient and cost effective way to use biomass for energy is to burn it for electricity. If it's too expensive to do that, then it will be too expensive for anything."

      Very true. Also true: the most efficient and cost effective way to use oil is to burn it. Oil refinement consumes a large amount of energy. Following this "logic," refined gasoline is a waste of energy, and crude oil should be used as an energy source.

      If I'm beginning to loose you, good. These "truths" don't support the conclusion that you are trying to make.

      Efficiency is not the only criteria that is used to judge an energy source. Burning biomass would not produce the kind of transportation fuel that our economy requires. Furthermore, it wouldn't produce the kind of responsible energy that our environment requires.

      4% is a starting point. Considering the technology is in its infancy, lets not prematurely judge its potential.

      -Drew
      Zymetis
      Rate this comment: 12345

      zymetis
      09/10/2008
      Posts:1
      Avg Rating:
      5/5
      • Re: Inaccuracies
        I get what you are trying to say, but your point is not made. Gasoline is a far superior fuel to crude oil. Oil has to be refined to yield the quality products we need from it. Contrary to your assertion, burning crude oil is not putting it to good use. It would be like putting sticks and twigs in a gas tank. Refining oil is not expensive. It adds a small percentage to the cost of refined products, which have been inexpensive to begin with.

        My point was this. Biomass has much useful energy. It takes far less money, energy, water and effort to process it into the quality energy product of electricity than any proposed process to make ethanol. Burning also wastes far less of the original energy and produces far less of a waste stream than cellulosic processes.

        Many people probably do not realize that electricity from biomass has recently become the single largest source of renewable energy in the U.S.

        If a cellulosic ethanol process were developed that managed to be highly efficient and overcame the substantial issues that confront it, I'd be excited about that. But, this particular news is fairly small and esoteric, not a step closer to a solution.
        Rate this comment: 12345

        MakeSense
        09/11/2008
        Posts:99
        Avg Rating:
        3/5
        • Re: Inaccuracies
          Electricity is pure thermodynamic work. Even so, it has limitations. We don't yet have a convenient and inexpensive way to store it for transportation. Battery technology has been evolving for the past 20 years, and still has a long way to go until the economics rival good ole' gasoline.

          Liquid fuels are likely to be important for transportation for the foreseeable future.

          You can burn biomass to make electricity, cleanly, and relatively cheaply. You just can't use it to power a car or an airplane.
          Rate this comment: 12345

          ChuckInReno
          09/17/2008
          Posts:19
          Avg Rating:
          3/5
    • Re: Inaccuracies
      not all of the inaccuracies cited are as extreme as they might appear at first glance.

      most ethanol in the u.s. comes from corn. a non-trivial amount (mostly what we drink) comes from barley, grapes and the like. a very small amount currently comes from cellulosic processes.

      most of the research going on now concentrates on single-microbe processes.  when suitable candidates are identified they may be able to be combined to create a single process that combines pre-processing with ethanol production.  while it's true that current production is in the 4% range, it's not unreasonable to predict higher yields in the future.

      many of the microbes being studied are found in nature.  the microbe described here has been modified to turn off one metabolic pathway (producing lactic acid) so that another existing pathway (producing ethanol) is used more.  that's a lot different (and a lot less scary) than adding significant new functionality.  and, they often work at temperatures that are rare in nature.

      burning complex organics often yields undesirable byproducts that would have to be removed from the exhaust.  this would significantly increase the cost of electricity production.
      Rate this comment: 12345

      jackofalltra...
      09/11/2008
      Posts:1
      • Re: Inaccuracies
        Sources of ethanol include a few million gallons from brewery waste include collected VOCs, such as at Coors Brewery in CO, fermented whey, and other recovered fermented sugars, both in CA, and other minor non-corn starch sources, and a bit from biomass. Scientists say "most" to be accurate as opposed to "virtually all" which is inaccurate.
        Re: propanol and other odd numbered carbon alcohols, biology is very good at making even numbered carbon compounds (beside C1) due to the acetyl (C2) building block, so biology makes ethanol, butanol, and even numbered fatty acids easily, but not odd numbered one easily. Adding a chemical step adds $$$.

        Making electricity from biomass works but is costly due low energy density compared to coal and slagging (mineral deposits) in boiler, not to mention the separate issue of <10% energy efficiency of electricity. Still, on the 7th anniversary of the 9-11 attack, we must not forget we were attacked, as stated by the attackers prior to their suicidal mission, to convincee the American public to get the US out of the Middle East. We are there due to their massive petroleum reserves, so one critical key to minimizing the next attack on our society is to decrease our current need to purchase over 60% of our petroleum needs from outside the US, much from countries that support terrorists aimed destroying us. Making electricity does little since most of our petroleum use goes to transportation using liguid fuels. Given this situation, I am amazed constantly by those who criticize attempts to displace petroleum use but have no solution. Also some provide solutions that does not address the problem. Biomass to electricity is the latter, so thanks for positive input. Our society is at risk so stop nit picking and provide constructive, helpful support to research pioneers.
        Rate this comment: 12345

        stagshaw
        09/11/2008
        Posts:1
        Avg Rating:
        3/5
        • Re: Inaccuracies
          Are you saying or implying that one cannot point out a few inaccuracies within an article without being obliged to first solve our energy problems? I strongly disagree.

          As a matter of energy policy, we ought to make the best use of the energy sources that we have available. If biomass can provide a large amount of energy as electricity as can most of the renewable sources at our disposal, then it doesn't take a major leap to imagine that the solution to imported oil for the transportation sector might just be electric cars. I'm personally hopeful that hybrids and on up to fully electric vehicles will mature soon.

          Don't be overly encouraged by any replacement of gasoline though. Only 42% of our oil demand goes to transportation. If we eliminated the need for gasoline and diesel entirely, we'd still import a significant amount of oil.
          Rate this comment: 12345

          MakeSense
          09/12/2008
          Posts:99
          Avg Rating:
          3/5
          • Re: Inaccuracies
            It's good to point out inaccuracies and exaggerations.  Presumably the main aim of ethanol production is liquid fuel for transporation. The engery recovery, e.g. compared to the solar energy available in the land area used is probably miniscule.

            Yes, buring the stuff would be more efficent.  All we need is some electric cars to make burning a far better choice than making ethannol.
            Rate this comment: 12345

            TooMany
            09/14/2008
            Posts:58
            Avg Rating:
            4/5
    • Re: Inaccuracies
      Cost of feedstock in corn-based ethanol represents around 70% of the overall cost. While distillation is certainly a cost issue with ethanol, and with cellulosic ethanol, pre-treatment technology (in biochemical based systems only) is also a cost issue. However, as the company Coskata claims, they can produce cellulosic ethanol at around $1/litre. It should be apparent to the many in the biofuels sector, that cellulosic ethanol is basically a stop gap measure until the commercialisation of advanced biofuels (e.g. hydrocarbons from LS9, Virent, Amyris Biotechnologies and biobutanol from Gevo, BP-Dupont) which are currently shifting from lab to pilot project phase. Cellulosic ethanol production is currently moving from pilot to commercial scale.
      Rate this comment: 12345

      arvindkc77
      09/15/2008
      Posts:2
  • Wrong Side of the Bed
    Thank you Mr. Patel-Predd for trying to make Ethanol better. I am quite sure that you have given up a lot of your own time to get this far.  Hopefully you can ignore the negativity expressed here by some of the posters.

    After spending 40 years in business I find nothing wrong with a difference of opinion followed by at least a suggestion or recommendation for change because that's what get's stuff done.  Everyone can learn from working together as a team but negativity followed by more negativity just makes most people not even want to listen.

    Tom G
    tomgarven@hotmail.com
    Rate this comment: 12345

    tomgarven
    09/15/2008
    Posts:16
    Avg Rating:
    4/5
    • Re: Wrong Side of the Bed
      I don't understand your meaning. Are you trying to say that there is no room for correction or contradictory evidence in a forum like this without it being branded "negativity"?

      Try to see all comments as coming from the point of view of attempting to arrive at a genuine solution. That's a positive thing! It's not meant to be negative at all.
      Rate this comment: 12345

      MakeSense
      09/16/2008
      Posts:99
      Avg Rating:
      3/5

Log In

Forgot your password?     Register »
Advertisement

Videos

Malleable Maps, Artistic Robots and Bubble Interfaces
Technology Review January/February 2010

Current Issue

Security in the Ether
Information technology's next grand challenge will be to secure the cloud--and prove we can trust it.
Advertisement
Advertisement
Subscribe to Technology Review's daily e-mail update. Enter your e-mail address

TECHNOLOGY RESOURCES

More Technology News from Forbes

Advertisement
MIT Massachusetts Institute of Technology © 2010 Technology Review. All Rights Reserved.