Her latest strategy is a computational approach that can rapidly identify thousands of new protein sequences for screening. This approach generates many more sequence variants than other methods do, greatly increasing the chances of creating functional molecules with useful new properties.
Arnold is using the technique to build libraries containing thousands of new cellulase genes. She and her colleagues will then screen the cellulases to see how they act as part of a mixture of enzymes. "If you test them simply by themselves, you really don't know how they work as a group," she says.
To fulfill her ultimate goal of a superbug able to feed on cellulose and produce biofuels, Arnold is working with James Liao, a professor of chemical engineering at the University of California, Los Angeles. Liao recently engineered E. coli that can efficiently convert sugar into butanol, a higher-energy biofuel than ethanol. Arnold hopes to be able to incorporate her new enzymes into Liao's butanol-producing microbes. Gevo, a startup cofounded by Arnold and based in Denver, CO, has licensed Liao's technology for use in the large-scale production of advanced biofuels, including butanol.
Overcoming cellulose's natural resistance to being broken down is "one of the most challenging protein-engineering problems around," says Arnold. Solving it will help determine whether low-emission biofuels will ever be a viable substitute for fossil fuels.

See All 10 Emerging Technologies 2008
Comments
mkogrady on 02/19/2008 at 3:09 PM
127
Nature has been feeding itself for ions, are we trying to reinvent the wheel or just increase the effciency?
ronwagn on 02/20/2008 at 6:43 AM
10
I do fear that we might genetically produce some organism that will destroy live plants in the environment on a large scale. I am pretty sure we have no oversight on this possibility. Do a search on grey goo. Grey goo is the scenario where nanotechnology destroys all living things.
jgillece on 02/20/2008 at 11:50 AM
2
http://www.wired.com/science/planetearth/magazine/15-10/ff_plant?currentPage=4
mkogrady on 02/29/2008 at 2:35 PM
127
my 2 cents
mko
devassocx on 02/20/2008 at 1:25 AM
29
cellulose ethanol will significantly reduce the
usage of gasoline.
From what I have read 'significant' is less than
3.5% of our gasoline usage. I don't see this as
significant.
The only thing significant is the terrific increase in food prices that has resulted from
using corn to make ethanol. Typical gov't
action.
ronwagn on 02/20/2008 at 6:30 AM
10
martinaatayo on 02/20/2008 at 8:46 PM
37
in the methodology, fermentation process time
factor itself to achieve desired large scale industrial product commensurate to daily user demand, comparable to existing classical thermal petro-chemical/petroleum product synthesis, even on readily availability of basic
raw materials, raises doubts on this process'
potential to eventually replace industrail scale fuel energy in today's world. very good but to keenly follow the progress.
MakeSense on 02/21/2008 at 9:46 AM
79
deep on 02/25/2008 at 3:46 AM
1
Scottar on 03/10/2008 at 12:01 AM
7
http://www1.eere.energy.gov/biomass/ethanol_myths_facts.html
So I can only wonder where DOE gets it's input from?
ronwagn on 03/10/2008 at 1:28 PM
10
Gcanno on 02/25/2008 at 3:53 AM
7
The Definition of an expert is a person that avoids all the little mistakes on the way to the big fallacy.
Pete Seeger
ronwagn on 03/10/2008 at 1:30 PM
10
Arachnid on 03/09/2008 at 10:56 AM
1
mkogrady on 03/19/2008 at 3:26 PM
127
skipcjr on 03/16/2008 at 7:24 PM
6
terrys122 on 03/19/2008 at 5:50 PM
1
anorlunda on 06/22/2008 at 4:31 PM
1
In order for an item to be "likely to affect our lives in the future", it must not only have great promise if successful, it must be highly likely to succeed in the research, development, production, deployment and competition phase of a life cycle. Anything in preliminary or in the early stages should be disqualified.
Disagree? Just think of all the exciting ideas in the past 40 years for technologies that promised to displace silicon as king of the hill in electronics. There must have been 2 or 3 such exciting preliminary ideas every year.
If TR wanted to make this top 10 list truly significant and respected, it should make the selection process transparent, or open it up to peer voting on the web. Perhaps a wiki on "Top 10" might be the way to go.
jpdemers on 11/10/2008 at 11:11 PM
34