Friday, December 15, 2006

New Hope for Stem-Cell Therapy

Stem cells derived via asexual reproduction could provide an efficient way to generate cells that are tissue-matched to the donor.

By Emily Singer

Scientists have created embryonic stem-cell lines that are a perfect tissue match to their mouse donors, allowing the cells to be transplanted without immune rejection. If similar methods succeed in humans, the technique could provide a way to generate patient-matched cells for transplant that doesn't require destruction of viable human embryos. "This presents a much more efficient way … of generating tissue-matched embryonic stem cells," says George Daley, a physician and researcher at Children's Hospital Boston and lead scientist on the project.

Scientists one day hope to use stem-cell-derived therapies to replace lost or diseased cells, such as in Parkinson's disease or sickle-cell anemia. But just as with organ transplants, these cells must be closely matched to the recipient's genetic profile in order to avoid immune rejection. Currently, the best method for generating genetically matched cells is cloning or nuclear transfer, in which an adult cell from the donor is inserted into an egg that has had its DNA removed, generating an embryo genetically identical to the one from the adult donor. In 2002, Daley and collaborators showed that customized stem cells generated via cloning could be implanted in mice to cure genetic deficits.

But cloning, which often requires a large number of eggs, is a technically challenging and inefficient way to make stem cells, and it raises ethical objections because it requires destruction of a human embryo. An alternative is to generate cells using parthenogenesis, a form of asexual reproduction in which an embryo can develop without fertilization. Parthenogenesis occurs naturally in some plants and vertebrates, but not in mammals. However, scientists can trick mammalian eggs into undergoing parthenogenesis by activating the egg as if it had been fertilized. To date, such embryos have been generated in mice, primates, and even humans. Human versions of these embryos are called parthenotes; they survive only a few days and are therefore unable to develop into a human life.